New Zealand Journal of Agricultural Research abstracts

---

Development and evaluation of an enzyme immunoassay for sporidesmin in pasture

R. G. COLLIN
L. R. BRIGGS
N. R. TOWERS

AgResearch
Ruakura Agricultural Centre
Private Bag 3123
Hamilton, New Zealand

Abstract  An enzyme immunoassay was developed which could detect sporidesmin in pasture samples. The assay had a linear working range of 0.4-40 ng/ml sporidesmin A while validation using pasture extracts gave mean percentage recoveries of 124% for samples containing 1 ng/ml sporidesmin and 97% for extracts containing 20 ng/ml sporidesmin. The sporidesmin levels and numbers of Pithomyces chartarum spores from many pasture samples were compared. The amount of sporidesmin detected generally corresponded to the number of spores found in the sample.

Keywords  ELISA; enzyme immunoassay; pasture; Pithomyces chartarum; sporidesmin

New Zealand Journal of Agricultural Research, 1995, Vol. 38: 297-302

0028-8233/95/3803-0297 $2.50/0 (c) The Royal Society of New Zealand 1995

PDF file of entire paper: medium quality (765K); (scanned from paper original: notes about this process)

---

This year's abstracts | Journal home page | All abstracts | Publishing home page