Abstract Freezing in leaves of N. menziesii was monitored by experimental freezing of attached twigs and seedlings growing naturally in a forest site and compared with a routine laboratory test in which excised plant material was used. Major differences in ice nucleation were observed: ice formed in attached leaves in situ at temperatures that were on average 4deg.C higher than in excised leaves, in both seedlings and adult trees. Excision did not affect the symptoms of frost damage. Mean foliar frost resistance was 2deg.C lower in seedlings than in mature shoots, and about 1deg.C lower in shoots measured in situ than in excised shoots.
Keywords Nothofagus; broad-leaved evergreen tree; differential thermal analysis (DTA); supercooling; ice nucleation; frost resistance
B96014
Received 16 February 1996; accepted 7 November 1996
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