New Zealand Journal of Zoology abstracts

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Short communication
Surface sterilisation of Heterodera trifolii Goffart (Nematoda: Tylenchida) and its monoxenic culture on root cultures of white clover (Trifolium repens L.)

F. S. HAY

AgResearch Grasslands Research Centre
Private Bag 11008
Palmerston North, New Zealand

Abstract  Various disinfectants and antibiotics were tested for their ability to eliminate contaminant organisms from second stage juveniles (J2) of clover cyst nematode (Heterodera trifolii Goffart). Sterile J2 were produced by alternating treatments of dilute Hibitane (chlorhexidine gluconate 1% w/v, isopropyl alcohol 0.8%) or Dettol (1% w/v chlorxylenol) with antibiotic (0.1% w/v Penicillin-G/Streptomycin sulphate BP). A convenient method involved alternate treatments with Hibitane (15 min), antibiotic (16 h), and Hibitane (15 min) with sterile water rinses after each treatment. Following sterilisation, H. trifolii was successfully reared on root cultures of white clover (Trifolium repens L.) grown on White's medium. Some 21-24% of sterilised juveniles formed egg-containing adult females on root cultures.

Keywords  clover cyst nematode; gnotobiotic culture; sterilisation; Heterodera trifolii

PDF file of entire paper: medium quality (204K); (scanned from paper original: notes about this process)

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